What are the mechanisms that regulate axon growth during the development and regeneration of mammalian neurons? Axons serve as vital communication channels within the nervous system, connecting neurons in the brain to the rest of the body and maintaining overall nervous system function. While young neurons demonstrate a remarkable capacity for regeneration and repair following injury, adult neurons often fail to regenerate, leading to permanent and irreversible damage to the nervous system. My research seeks to answer critical questions about this phenomenon: What molecular pathways are responsible for the decline in regenerative capacity as neurons mature? What regulatory mechanisms influence axon growth during development? Is the successful regeneration of axons in adult neurons dependent on replicating developmental mechanisms, or are there alternative pathways that facilitate repair? I address these questions through an integrated approach that combines functional genomics—including single-cell RNA-Seq, ATAC-Seq, and CUT&RUN—along with in vitro growth assays, in vivo mouse injury models, and behavioral assessments.